An essential RNA element resides in a central region of hepatitis E virus ORF2
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منابع مشابه
An essential RNA element resides in a central region of hepatitis E virus ORF2
Hepatitis E virus (genus Hepevirus, family Hepeviridae) is one of the most important causes of acute hepatitis in adults, particularly among pregnant women, throughout Asia and Africa where mortality rates can be 20-30 %. Hepatitis E virus has a single-stranded positive-sense RNA genome that contains three translated ORFs. The two 3' ORFs are translated from a subgenomic RNA. Functional RNA ele...
متن کاملThe ORF2 protein of hepatitis E virus binds the 5' region of viral RNA.
Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It is a plus-strand RNA virus with a 7.2-kb polyadenylated genome consisting of three open reading frames, ORF1, ORF2, and ORF3. Of these, ORF2 encodes the major capsid protein of the virus and ORF3 encodes a small protein of unknown function. Using the yeast three-hybrid system and traditional biochemical techni...
متن کاملCloning and expression of hepatitis E virus ORF2 as a vaccine candidate
Introduction: Hepatitis E virus (HEV) is a fecal-oral transmitting virus which causes a chronic liver disease. ORF2 is an immunogen capsid protein of HEV that has been proposed to be used for Hepatitis E vaccine design. It is a 660-amino acid protein which includes an immunogenic region (residues 112-607). This protein has been expressed in complete and truncated forms, using different expressi...
متن کاملA C-terminal hydrophobic region is required for homo-oligomerization of the hepatitis E virus capsid (ORF2) protein
Hepatitis E virus (HEV) is the causative agent of hepatitis E, an acute form of viral hepatitis. The open reading frame 2 (ORF2) of HEV encodes the viral capsid protein, which can self-oligomerize into virus-like particles. To understand the domains within this protein important for capsid biogenesis, we have carried out in vitro analyses of association and folding patterns of wild type and mut...
متن کاملA truncated hepatitis E virus ORF2 protein expressed in tobacco plastids is immunogenic in mice.
AIM To cost-effectively express the 23-ku pE2, the most promising subunit vaccine encoded by the E2 fragment comprising of the 3'-portion of hepatitis E virus (HEV) open reading frame 2 (ORF2) in plastids of tobacco (Nicotiana tabacum cv. SR1), to investigate the transgene expression and pE2 accumulation in plastids, and to evaluate the antigenic effect of the plastid-derived pE2 in mice. MET...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 2013
ISSN: 0022-1317,1465-2099
DOI: 10.1099/vir.0.051870-0